Peptide Reconstitution
FAQ

It depends on your target dose and preferred draw size. Use the reconstitution calculator to get the exact volume for your vial and dose. Common starting point: 2mL BAC water per 5mg vial gives 2,500mcg/mL concentration.

Bacteriostatic water (BAC water) is sterile water containing 0.9% benzyl alcohol. The benzyl alcohol acts as a preservative that prevents bacterial growth in your reconstituted peptide solution. This extends shelf life to 4-6 weeks refrigerated. Regular sterile water has no preservative and must be used within 24 hours.

BAC water contains 0.9% benzyl alcohol (preservative). Stable for 4-6 weeks refrigerated after reconstitution. Sterile water has no preservative. Use within 24 hours. For most peptide protocols, BAC water is correct. Sterile water is used when benzyl alcohol is contraindicated.

Units = (target dose in mcg / concentration in mcg/mL) x syringe units per mL. Example: 250mcg dose, 2,500mcg/mL concentration, U-100 syringe = (250/2500) x 100 = 10 units. The calculator does this automatically.

No. Shaking can cause foaming and may degrade peptide bonds. Instead, gently swirl the vial in a circular motion until the powder fully dissolves. The solution should be completely clear.

With BAC water, refrigerated at 2-8 degrees C: most peptides are stable for 4-6 weeks. Frozen at -20 degrees C: typically 6-12 months. Avoid repeated freeze-thaw cycles as this can degrade the peptide.

A clear solution is the goal. Cloudiness can indicate incomplete dissolution (give it more time to dissolve with gentle swirling) or potential contamination (do not use if contamination is suspected). Some peptides may have a slight yellow tint — this is normal for certain compounds.

Refrigerate at 2-8 degrees C. Keep away from light. Do not freeze unless storing for more than 6 weeks. Label the vial with the reconstitution date and concentration so you always know when it was mixed and what dose to draw.

No — angle the needle against the glass and let the water run down the side of the vial. Spraying directly onto the powder can cause foaming and may damage the peptide.

U-100 insulin syringes (29-31 gauge) are standard for most peptide protocols. The gauge determines needle thickness — finer is more comfortable for subcutaneous injections. Length: 5/16 inch (8mm) is typical for subcutaneous. Use the calculator to confirm your draw volume works with a U-100 syringe.

Verify with the calculator: concentration (mcg/mL) = peptide amount in mg x 1,000 / BAC water volume in mL. If you added 2mL to a 5mg vial: 5,000mcg / 2mL = 2,500mcg/mL. Track your vial in Protocol to catch any math errors before they affect your dosing.

Yes. peptide tracker saves your vial details — peptide amount, BAC water added, concentration — and automatically calculates your draw units for every dose. It tracks remaining doses and sends low-stock alerts before you run out. Free to download, 14 days of Pro automatically at install.

Any lyophilized peptide: BPC-157, TB-500, CJC-1295, Ipamorelin, Semaglutide, GHK-Cu, Semax, Selank, PT-141, Epithalon, and all others. The math is the same regardless of compound — peptide amount divided by BAC water volume.

Protocol's 81-compound research library at peptide compound research library covers dosing, half-life, reconstitution guidance, and research citations for every compound.

Use a sharps container. Never dispose of needles in regular trash. Many pharmacies accept used sharps containers. Check your local municipality for disposal options.